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Updated: Dec 27, 2025

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
Published on: May 23, 2014
An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy
Taishi Kakizuka1, Akira Takai, Keiko Yoshizawa
1Laboratory for Comprehensive Bioimaging, RIKEN Center for Biosystems Dynamics Research (BDR), 6-2-3 Furuedai, Suita-Shi, Osaka 565-0874, Japan. tomowatanabe@riken.jp.
Abstract:
Fluorescent protein-based reporter systems are used to track gene expression in cells. Here, we propose a modified bioluminescence resonance energy transfer (BRET) reporter as a maturation-less reporter that utilizes a peptide-assisted complementation strategy. Using effective dimerized peptides obtained from library-versus-library screening with more than 4000 candidates, rapid activation of the reporter was achieved.

