Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.9K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.3K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The protocol of a prospective, multicenter, randomized, controlled phase III study evaluating different cycles of oxaliplatin combined with S-1 (SOX) as neoadjuvant chemotherapy for patients with locally advanced gastric cancer: RESONANCE-II trial.

BMC cancer·2021
Same author

[Efficiency of sperm donation: An analysis of 440 qualified sperm donors in Chongqing Human Sperm Bank].

Zhonghua nan ke xue = National journal of andrology·2020
Same author

The complete mitochondrial genome of the <i>Papilio paris</i> (Lepidoptera: Papilionidae).

Mitochondrial DNA. Part B, Resources·2020
Same author

Age and gender dependence of liver diffusion parameters and the possibility that intravoxel incoherent motion modeling of the perfusion component is constrained by the diffusion component.

NMR in biomedicine·2020
Same author

Acute invariant NKT cell activation triggers an immune response that drives prominent changes in iron homeostasis.

Scientific reports·2020
Same author

Development of a robust crystallization platform for immune receptor TREM2 using a crystallization chaperone strategy.

Protein expression and purification·2020
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Dec 27, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K

Illumination modulation for reflective and fluorescent separation.

Ying Fu, Yunhao Zou, Liheng Bian

    Optics Letters
    |February 29, 2020
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new illumination modulation technique to separate reflective and fluorescent signals using a single spectral image. This method utilizes high-frequency illumination and total variation regularization for robust, noise-resistant performance.

    More Related Videos

    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.2K
    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
    07:34

    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

    Published on: August 22, 2019

    8.3K

    Related Experiment Videos

    Last Updated: Dec 27, 2025

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K
    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.2K
    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
    07:34

    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

    Published on: August 22, 2019

    8.3K

    Area of Science:

    • Optics
    • Image Processing
    • Spectroscopy

    Background:

    • Distinguishing between reflective and fluorescent signals is crucial in various imaging applications.
    • Existing methods often require multiple spectral images or complex setups.
    • High-frequency illumination can enhance separation but presents technical challenges.

    Purpose of the Study:

    • To introduce a novel, simplified method for reflective and fluorescent separation.
    • To demonstrate the feasibility of using a single spectral image for this task.
    • To develop a robust technique applicable to both simulated and real-world data.

    Main Methods:

    • Implementation of a unique illumination modulation system using readily available components.
    • Generation of high-frequency illumination tailored for reflective-fluorescent separation.
    • Application of total variation regularization to leverage spectral-spatial correlations and improve noise resilience.

    Main Results:

    • Successful separation of reflective and fluorescent components from a single spectral image.
    • Demonstration of the system's effectiveness with both simulated and experimental datasets.
    • Validation of the method's robustness against noise through regularization.

    Conclusions:

    • The proposed illumination modulation method offers an effective and practical solution for reflective and fluorescent signal separation.
    • The use of a single spectral image and off-the-shelf components simplifies the process.
    • The technique shows significant promise for diverse imaging and sensing applications.