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Updated: Dec 27, 2025

08:51
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
Published on: September 15, 2020
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How many cells are enough for single-cell infrared spectroscopy?
Yadi Wang1, Wentao Dai, Yue Wang
1Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Science, Shanghai 201800, China. lujunhong@zjlab.org.cn.
Abstract:
The influence of cell number on spectral variability among two different cell types and two states was quantitatively analyzed and evaluated by calculating the similarity distance of the single-cell spectral data obtained from synchrotron infrared microspectroscopy. We found that at least 15 cells are required to achieve robust results with 95% confidence.

