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Updated: Dec 27, 2025

Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
Published on: July 26, 2022
A Technical Introduction to Transmission Electron Microscopy for Soft-Matter: Imaging, Possibilities, Choices, and
Linda E Franken1, Kay Grünewald1, Egbert J Boekema2
1Department of Structural Cell Biology of Viruses, Heinrich-Pette Institute-Leibniz-Institute of Experimental Virology University of Hamburg, Centre for Structural Systems Biology, Notkestraße 85, 22607, Hamburg, Germany.
Abstract:
With a significant role in material sciences, physics, (soft matter) chemistry, and biology, the transmission electron microscope is one of the most widely applied structural analysis tool to date. It has the power to visualize almost everything from the micrometer to the angstrom scale. Technical developments keep opening doors to new fields of research by improving aspects such as sample preservation, detector performance, computational power, and workflow automation. For more than half a century, and continuing into the future, electron microscopy has been, and is, a cornerstone methodology in science. Herein, the technical considerations of imaging with electrons in terms of optics, technology, samples and processing, and targeted soft materials are summarized. Furthermore, recent advances and their potential for application to soft matter chemistry are highlighted.
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