INVESTIGATION OF STRAINS OF MICROORGANISMS ISOLATED FROM CHILDREN WITH RECURRENT TONSILLITIS

Chronic and recurrent tonsillitis associated with tonsillitis recurrence, which can greatly affect the quality of life of the patient. Among the causes of chronic infection and resistance to antibiotic treatment are the formation of biofilms in the respiratory tract in respiratory infections. The purpose of the study is to identify the ability to biofilm strains of streptococci on the surface of the epithelial tissue of the oropharynx isolated from patients with recurrent tonsillitis. Clinical strains of Streptococcus spp bacteria obtained from the oropharynx of 22 children with recurrent tonsillitis were investigated. The biofilm formation ability was studied by growing bacteria on 96-well plates. It was found that 9 (40.09%) strains out of 22 had film-forming ability, among which 1 (4.54%) strains showed strong film-forming, 4 (18.18%) strains-moderate, 4 strain (18.138%). - weak film formation and in 13strains (59.09%) this ability was absent. Studies of the intensity of biofilm formation over three days of cultivation showed a tendency to increase the rate of optical density (OD) in the line: 0,1691±0,019 on the first day (24 h); 0,2159±0,029 in the second (48 h) and in the third - 0,3156±0,055. The degree of severity of the film-forming ability of the studied strains of streptococci isolated from patients with recurrent tonsillitis showed an increase in the optical density (OD) over three days of cultivation, with it fluctuating within 0,1407