Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.8K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.8K
Membrane Domains01:18

Membrane Domains

6.9K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
6.9K
COP Coated Vesicles00:59

COP Coated Vesicles

16.7K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
16.7K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.0K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.0K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.7K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.7K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

9.0K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mural cells protect the adult brain from hemorrhage but do not control the blood-brain barrier in developing zebrafish.

eLife·2026
Same author

The formation, dynamics, and disassembly of caveolae.

Current opinion in cell biology·2026
Same author

Mitochondrial fission mediates an evolutionarily conserved antibacterial defense response.

Science immunology·2026
Same author

A lipid-centric view of endocytosis by caveolae.

Nature cell biology·2026
Same author

Nisin ZP Combined with Limited Chemotherapy in Oropharyngeal Squamous Cell Carcinoma: A Case Report.

Archives of clinical and medical case reports·2026
Same author

Feeding-regulated glycogen metabolism drives rhythmic liver protein secretion.

Nature metabolism·2026
Same journal

Mechanosensing in immune cells: Implications for migration and beyond.

Current opinion in cell biology·2026
Same journal

Emerging role of organelles in cell migration.

Current opinion in cell biology·2026
Same journal

Nuclear adaptation in cell migration.

Current opinion in cell biology·2026
Same journal

Patterns in motion: Choreographing dynamic cell behaviours during tissue repair.

Current opinion in cell biology·2026
Same journal

Quo vadis reconstituted cell surfaces? Purpose and future perspectives for minimal systems of the cell plasma membrane.

Current opinion in cell biology·2026
Same journal

Nuclear determinants of mRNA and protein isoforms.

Current opinion in cell biology·2026
See all related articles

Related Experiment Video

Updated: Dec 26, 2025

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.2K

Caveolae: Formation, dynamics, and function.

Robert G Parton1, Kerrie-Ann McMahon2, Yeping Wu2

  • 1The University of Queensland, Institute for Molecular Bioscience, Brisbane, Queensland, 4072, Australia; The University of Queensland, Centre for Microscopy and Microanalysis, Brisbane, Queensland, 4072, Australia.

Current Opinion in Cell Biology
|March 9, 2020
PubMed
Summary
This summary is machine-generated.

Caveolae, cell surface pits, form via caveolin proteins and lipids. These structures protect cells and aid signaling, with disassembly triggered by membrane tension or UV stress.

Keywords:
CaveolaeCaveolinCavinEHD2LipidMembrane traffic

More Related Videos

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
11:42

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

Published on: January 22, 2015

19.7K
Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

80.8K

Related Experiment Videos

Last Updated: Dec 26, 2025

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.2K
Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
11:42

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

Published on: January 22, 2015

19.7K
Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

80.8K

Area of Science:

  • Cell biology
  • Membrane biophysics
  • Molecular mechanisms

Background:

  • Caveolae are flask-shaped invaginations of the plasma membrane.
  • They are formed by caveolin proteins and cavin proteins interacting with membrane lipids.
  • Caveolae play roles in membrane trafficking, signal transduction, and mechanoprotection.

Purpose of the Study:

  • To review recent mechanistic studies on caveolae formation.
  • To elucidate the functional properties of caveolae as protective and signaling structures.
  • To discuss the dynamic nature of caveolae, including their assembly and disassembly.

Main Methods:

  • Review of recent mechanistic studies.
  • Analysis of protein-lipid interactions in caveolae formation.
  • Investigation of stimuli-induced caveolae disassembly.

Main Results:

  • Caveolae assembly involves caveolin proteins forming a platform with membrane lipids.
  • Cavin proteins contribute to the formation of the caveolae structure.
  • Caveolae disassembly can be induced by increased membrane tension or stress stimuli like UV radiation.

Conclusions:

  • Caveolae are dynamic structures with versatile functions.
  • Their formation and disassembly are regulated by specific protein-lipid interactions and environmental cues.
  • Caveolae serve as crucial protective and signaling hubs within the cell.