[Expression and function characterization of Gimap5 gene from different breeds of broilers]

Area of Science:

• Immunology
• Genetics
• Poultry Science

Background:

• GTPase immune-associated protein 5 (Gimap5) is vital for T cell homeostasis and immune functions.
• Its specific roles and characteristics in poultry remain largely uncharacterized.

Purpose of the Study:

• To clone and analyze the Gimap5 gene in broilers.
• To investigate the tissue expression and distribution of Gimap5.
• To determine the functional role of Gimap5 in inflammatory responses in poultry.

Main Methods:

• Cloning of the full-length Gimap5 coding sequence using RT-PCR.
• Bioinformatic analysis of the gene sequence.
• Quantitative real-time PCR (RT-qPCR) for tissue expression and inflammatory response analysis.

Main Results:

• The Gimap5 gene sequence (771 bp, 256 amino acids) showed low conservation across species.
• Gimap5 was widely expressed across tissues, with similar patterns in AA⁺ and Sanhuang broilers, though expression levels varied.
• Inflammatory response led to Gimap5 downregulation in blood and liver but upregulation in the bursa of Fabricius.

Conclusions:

• Gimap5 is a multifunctional gene involved in poultry development and inflammatory responses.
• The gene's distinct expression changes during inflammation suggest its potential as a diagnostic marker for inflammatory conditions in poultry.