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Circular RNA has_circ_0003204 inhibits oxLDL-induced vascular endothelial cell proliferation and angiogenesis

Huifang Liu1, Xirui Ma1, Ziming Mao1

  • 1Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Cellular Signalling
|March 11, 2020
PubMed

Insights

Circular RNAs (circRNAs) are crucial in atherosclerosis. This study shows hsa_circ_0003204 promotes endothelial cell damage, suggesting it

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Cell Biology

Background:

  • Circular RNAs (circRNAs) are prevalent in eukaryotes and implicated in atherosclerosis.
  • Endothelial cell dysfunction is central to atherosclerotic plaque development.

Purpose of the Study:

  • To investigate the role of hsa_circ_0003204 in atherosclerosis.
  • To explore the functional impact of hsa_circ_0003204 on human umbilical vein endothelial cells (HUVECs) under ox-LDL stimulation.

Main Methods:

  • Investigated hsa_circ_0003204 expression in ox-LDL-induced HUVECs.
  • Performed knockdown of hsa_circ_0003204 to assess its effects.
  • Analyzed cell proliferation, migration, invasion, and apoptosis.
  • Examined the expression of key endothelial-mesenchymal transition markers (E-cadherin, N-cadherin, vimentin).

Main Results:

  • hsa_circ_0003204 was overexpressed in ox-LDL-treated HUVECs.
  • Knockdown of hsa_circ_0003204 enhanced HUVEC proliferation, migration, and invasion.
  • hsa_circ_0003204 knockdown reduced ox-LDL-induced HUVEC apoptosis.
  • Reduced E-cadherin and increased N-cadherin and vimentin expression were observed upon hsa_circ_0003204 knockdown.

Conclusions:

  • hsa_circ_0003204 significantly impacts HUVEC proliferation and angiogenesis.
  • hsa_circ_0003204 is implicated in endothelial cell damage during atherosclerosis.
  • hsa_circ_0003204 represents a potential therapeutic target for atherosclerosis-related endothelial dysfunction.

Keywords:
AtherosclerosisCircular RNAscirc_0003204

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