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Related Experiment Videos

Forskolin and phorbol esters decrease the same K+ conductance in cultured oligodendrocytes.

B Soliven1, S Szuchet, B G Arnason

  • 1Department of Neurology, University of Chicago, Illinois 60637.

The Journal of Membrane Biology
|October 1, 1988
PubMed
Summary
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Forskolin and phorbol 12-myristate 13-acetate (PMA) modulate outward potassium currents in cultured ovine oligodendrocytes (OLGs). These findings suggest distinct second-messenger systems regulate OLG potassium currents, impacting myelin development.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Cultured ovine oligodendrocytes (OLGs) develop voltage-dependent potassium currents, including outward and inward rectifier currents, crucial for their function.
  • Oligodendrocytes are vital for myelin sheath formation in the central nervous system.
  • Myelinogenic metabolism in OLGs can be altered by compounds affecting intracellular signaling pathways.

Purpose of the Study:

  • To investigate the effects of forskolin and phorbol 12-myristate 13-acetate (PMA) on outward potassium currents in cultured ovine oligodendrocytes.
  • To explore the roles of distinct second-messenger systems in modulating these potassium currents.

Main Methods:

  • Electrophysiological recordings were used to measure outward potassium currents in cultured ovine oligodendrocytes.

Related Experiment Videos

  • Oligodendrocytes were treated with forskolin (adenylate cyclase activator) and PMA (protein kinase C activator).
  • Effects were assessed under varying concentrations, with inhibitors (adenosine, psychosine), and controlled intracellular calcium levels.

    • Main Results:

    • Forskolin significantly decreased steady-state outward current amplitude and altered inactivation time course in a dose-dependent manner.
    • PMA also reduced outward current amplitude, with less impact on the decay phase compared to forskolin.
    • The effects of forskolin were attenuated by an adenylate cyclase inhibitor, while PMA's effects were inhibited by a protein kinase C inhibitor.

    Conclusions:

    • Cultured ovine oligodendrocytes' potassium currents are physiologically modulated by at least two distinct second-messenger systems.
    • These systems, involving cAMP and protein kinase C, may converge on common downstream targets.
    • Understanding these modulations is critical for comprehending oligodendrocyte function and myelin development.