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Updated: Dec 26, 2025
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
Published on: January 10, 2017
Amperometric biosensor based on coupling aminated laccase to functionalized carbon nanotubes for phenolics detection
Abdelmageed M Othman1, Ulla Wollenberger2
1Department of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknechtstrasse 24-25, 14476 Potsdam, Golm, Germany; Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 El Bohouth St., Dokki, 12622 Giza, Egypt.
A novel laccase biosensor was developed using functionalized carbon electrodes for detecting phenolic compounds. This sensitive and stable biosensor shows high efficiency for ABTS and catechol detection.
Area of Science:
- Biotechnology
- Electrochemistry
- Biosensor development
Background:
- Phenolic compounds are widespread environmental pollutants.
- Development of sensitive and selective biosensors is crucial for their detection.
Purpose of the Study:
- To develop and characterize a laccase-based biosensor for phenolic compounds.
- To optimize enzyme immobilization and electrode modification for enhanced performance.
Main Methods:
- Chemical modification of laccase and immobilization on functionalized screen-printed carbon electrodes (SPCEs).
- Utilized carboxyl functionalized multi-walled carbon nanotubes (COOH-MWCNT) for electrode modification.
- Electrochemical characterization and sensitivity assessment using various phenolic compounds.
Main Results:
- Optimized biosensor achieved highest response with aminated laccase adsorbed onto COOH-MWCNT modified SPCEs.
- High sensitivity and electrocatalytic efficiency observed for ABTS and syringaldazine.
- Biosensor demonstrated excellent stability and reusability, retaining significant activity after multiple cycles and long-term storage.
Conclusions:
- The developed laccase biosensor offers a promising platform for sensitive and stable detection of phenolic compounds.
- The strategy of enzyme amination and COOH-MWCNT functionalization enhances biosensor performance.
- This biosensor has potential applications in environmental monitoring and food safety analysis.
