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Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Related Experiment Video

Updated: Dec 26, 2025

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Published on: July 12, 2014

Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP-Tag Technologies.

Conner A Hoelzel¹, Xin Zhang¹

¹Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, PA 16802, USA.

Chembiochem : a European Journal of Chemical Biology

|March 18, 2020

Summary

Advances in self-labeling protein tags like HaloTag and SNAP-tag enable precise visualization and manipulation of cellular proteins. These tools offer new insights into cell physiology and biological pathways.

Keywords:

HaloTag SNAP-tag bio-orthogonal chemistry chemical biology molecular probes

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Last Updated: Dec 26, 2025

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

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Area of Science:

Cellular Biology
Biochemistry
Molecular Biology

Background:

Understanding cellular physiology relies on visualizing and manipulating protein behavior.
Biorthogonal protein labeling methods are essential tools for this purpose.
HaloTag and SNAP-tag are prominent self-labeling protein tag systems.

Purpose of the Study:

To review recent advancements in probe technology for self-labeling protein tags.
To highlight the applications of HaloTag and SNAP-tag systems.
To discuss methodologies for protein manipulation and biological pathway control.

Main Methods:

Review of small-molecule probes for fluorogenic imaging.
Application of super-resolution fluorescence microscopy techniques.
Exploration of methods for perturbing protein function.

Main Results:

Development of advanced small-molecule probes for no-wash imaging.
Enhanced capabilities for super-resolution microscopy using these probes.
Emerging methodologies for controlling biological pathways via protein manipulation.

Conclusions:

HaloTag and SNAP-tag systems are powerful tools for visualizing and manipulating biological processes.
These technologies provide unique scientific insights beyond traditional methods.
Expanding self-labeling tag systems will unlock new research frontiers in biology.