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Functional Plasma Polymerized Surfaces for Biosensing.

Ekaterina Makhneva1, Laura Barillas1, Zdeněk Farka2

  • 1RG Bio Sensing Surfaces, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany.

ACS Applied Materials & Interfaces
|March 24, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed water-stable, oxygen-rich plasma polymerized (pp) films for biosensor applications. These functional films enhance biomolecule immobilization, improving SPR immunosensor sensitivity, stability, and regenerability for biological analysis.

Keywords:
atmospheric-pressure plasma-induced polymerizationbiosensingchemical compositionfunctional polymersthin films

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Area of Science:

  • Biomolecular Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Biosensors offer fast, economical, and user-friendly analysis of complex samples.
  • Effective biomolecule immobilization requires a stable, reactive thin functional layer for biosensor applications.

Purpose of the Study:

  • To develop water-stable, oxygen-rich plasma polymerized (pp) films using atmospheric-pressure jet plasma.
  • To evaluate the efficacy of these pp films as matrix layers for Surface Plasmon Resonance (SPR) immunosensors.

Main Methods:

  • Atmospheric-pressure jet plasma was used to deposit three types of oxygen-rich pp films.
  • Characterization of the pp films was performed.
  • The pp films were utilized as matrix layers in SPR immunosensors.

Main Results:

  • The developed pp films demonstrated water stability and suitability for biomolecule immobilization.
  • SPR immunosensors employing these pp films exhibited excellent sensitivity, stability, and regenerability.
  • The pp films proved effective in maintaining biomolecule functionality within the sample matrix.

Conclusions:

  • Atmospheric pressure plasma-induced polymerization is a powerful method for creating robust matrix layers for biosensors.
  • The developed pp films offer a reliable and effective solution for biomolecule immobilization in SPR immunosensors.
  • This technique presents a versatile alternative for preparing functional layers across various biological applications.