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Vascular bifurcation mapping with photoacoustic microscopy.
Boudewijn van der Sanden1, Olivier Hugon2, Mehdi Inglebert2
1Université Grenoble Alpes, Platform of Intravital Microscopy, CNRS, Grenoble INP, INSERM, TIMC-IMAG, 38000 Grenoble, France.
Biomedical Optics Express
|March 25, 2020
Summary
Early detection of microvascular changes in cancer is crucial. Optical-Resolution Photoacoustic Microscopy (OR-PAM) enables quantitative mapping of vascular bifurcations, aiding in early cancer diagnosis.
Area of Science:
- Biomedical Optics
- Medical Imaging
- Cancer Diagnostics
Background:
- Early detection of microvascular changes is critical for cancer diagnosis.
- Vascular bifurcation density serves as a biomarker for tumor sprouting activity.
Purpose of the Study:
- To develop and validate a method for quantitative vascular bifurcation mapping.
- To assess the utility of Optical-Resolution Photoacoustic Microscopy (OR-PAM) for this application.
Main Methods:
- Utilized OR-PAM to acquire 3D images of hemoglobin distribution.
- Developed a Virtual Tubes out of Bifurcations (ViTuBi) approach to map bifurcations.
- Applied a 3D tubeness filter to identify and quantify bifurcations.
Main Results:
- Successfully mapped vascular bifurcations quantitatively in 2D.
- Demonstrated the creation of virtual tubes from bifurcations in OR-PAM image stacks.
- Established a basis for comparing this fast analysis to classical methods.
Conclusions:
- OR-PAM combined with ViTuBi offers a promising tool for functional analysis of vascular bifurcation density.
- This method facilitates easier implementation for analyzing vascular changes in healthy and diseased tissues.
- Potential for improved early cancer detection through microvascular analysis.
