Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

7.9K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.9K
X-ray Imaging01:24

X-ray Imaging

9.6K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
9.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Terminal-Group Engineering to Amplify Asymmetric Electronic Interactions in COFs for Enhanced Optoelectronic Performance.

Nano letters·2026
Same author

Structural fatigue failure analysis and lifetime reliability monitoring strategy of the lattice jib in all-terrain cranes.

Scientific reports·2026
Same author

Zero-Contrast Percutaneous Renal Denervation Guided by Multimodal Image Fusion.

JACC. Case reports·2026
Same author

Comment On "Pilot Study of Immediate Lymphatic Reconstruction (ILR) During Lymph Node Dissection for Node-Positive Melanoma: Feasibility, Safety, and Early Outcomes".

Journal of surgical oncology·2026
Same author

SOD1 lactylation impair its enzymatic activity by conformational change to aggravate intervertebral disc degeneration.

Nature communications·2026
Same author

ABSCISIC ACID-INSENSITIVE 5 (ABI5) Orchestrates Seasonal Growth Cessation and Wood Formation Inhibition in <i>Populus tomentosa</i>.

Plants (Basel, Switzerland)·2026
Same journal

From Geometry to Intensity: A Coarse-to-Fine Pipeline for Unsupervised 3D Ultrasound Stitching.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

AVA: Automated Viewability Analysis for Ureteroscopic Intrarenal Surgery.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Kidney Endoscopy Video to Preoperative CT Alignment for Depth Estimation.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Deep learning‑based cell type prediction in lung tissue from brightfield histology using CODEX-derived labels.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Reconstructing physiological signals from fMRI across the adult lifespan.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.

Proceedings of SPIE--the International Society for Optical Engineering·2026
See all related articles

Related Experiment Video

Updated: Dec 25, 2025

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.6K

High-resolution x-ray luminescence computed tomography.

Michael C Lun1, Changqing Li1

  • 1Department of Bioengineering, University of California, Merced, Merced, CA 95343, USA.

Proceedings of Spie--The International Society for Optical Engineering
|March 28, 2020
PubMed
Summary
This summary is machine-generated.

High-resolution X-ray luminescence computed tomography (XLCT) uses a focused beam for enhanced sensitivity. This advanced imaging technique successfully resolved small targets, paving the way for faster 3D XLCT scans.

Keywords:
optical imagingoptical tomographytomographic imagingx-ray imagingx-ray luminescence computed tomography

More Related Videos

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
10:42

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

Published on: June 16, 2016

9.6K
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.5K

Related Experiment Videos

Last Updated: Dec 25, 2025

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.6K
In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM
10:42

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography CT and Light Microscopy LM Correlated with Scanning Electron Microscopy SEM

Published on: June 16, 2016

9.6K
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

13.5K

Area of Science:

  • Biomedical imaging
  • Medical physics
  • Molecular imaging

Background:

  • High-resolution imaging is crucial for biomedical advancements.
  • X-ray luminescence computed tomography (XLCT) merges X-ray's spatial resolution with optical imaging's molecular sensitivity.
  • Previous XLCT methods using collimated X-ray beams had limitations in sensitivity and resolution.

Purpose of the Study:

  • To demonstrate the high-spatial resolution capabilities of a focused X-ray beam-based XLCT system.
  • To evaluate the system's ability to resolve small targets at depth.
  • To improve the XLCT system for faster 3D scanning.

Main Methods:

  • Utilized a focused X-ray beam as the excitation source for XLCT.
  • Embedded Gd2O2S:Eu3+ targets (200 µm and 150 µm) in tissue-mimicking phantoms.
  • Scanned and reconstructed transverse sections to assess target separation and accuracy.
  • Improved the XLCT system for 3D scanning feasibility.

Main Results:

  • Successfully demonstrated high-spatial resolution imaging with the focused X-ray beam.
  • Achieved accurate separation of 200 µm and 150 µm targets with good shape and location fidelity.
  • Confirmed the focused beam's ability to resolve targets at depth.
  • Enhanced the XLCT system for rapid 3D imaging.

Conclusions:

  • Focused X-ray beam XLCT offers superior spatial resolution and sensitivity compared to previous methods.
  • The developed system accurately visualizes small targets, crucial for molecular imaging applications.
  • The advancements enable faster 3D XLCT, expanding its utility in biomedical research.