Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differences in radiation response among human cervix carcinoma cell lines.

L R Kelland1, G G Steel

  • 1Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, U.K.

Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
|November 1, 1988
PubMed
Summary

Newly developed cervical cancer cell lines show significant variations in radiosensitivity and radiation recovery. These differences in intrinsic radiosensitivity could be valuable for predicting patient response to radiotherapy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bcl-2 family immunohistochemistry.

Methods in molecular medicine·2011
Same author

Guidelines for the welfare and use of animals in cancer research.

British journal of cancer·2010
Same author

Preclinical pharmacokinetics and metabolism of a novel prototype DNA-PK inhibitor NU7026.

British journal of cancer·2005
Same author

Small molecule anticancer drugs.

IDrugs : the investigational drugs journal·2005
Same author

Antitumor activity of imidazothioxanthones in murine and human tumor models in vitro and in vivo.

Anticancer research·2004
Same author

Of mice and men: values and liabilities of the athymic nude mouse model in anticancer drug development.

European journal of cancer (Oxford, England : 1990)·2004

Area of Science:

  • Radiobiology
  • Oncology
  • Cell Biology

Background:

  • Carcinoma of the cervix is a significant global health concern.
  • Understanding cellular responses to radiation is crucial for optimizing radiotherapy.
  • Variability in radiosensitivity among cancer cell lines necessitates detailed characterization.

Purpose of the Study:

  • To determine the radiobiological properties of five new carcinoma of the cervix cell lines.
  • To compare their survival curves at different radiation dose rates.
  • To investigate potential clinical applications of intrinsic radiosensitivity testing.

Main Methods:

  • In vitro clonogenic assay used to assess cell survival.
  • Comparison of survival curves at acute (150 cGy/min) and low dose rates (3.2 and 1.6 cGy/min).

Related Experiment Videos

  • Application of the Incomplete Repair model to analyze radiation recovery kinetics.

    • Main Results:

    • Significant differences in acute radiosensitivity and low dose-rate sparing observed among the five cell lines.
    • Two lines (HX151c, HX160c) were radiosensitive with limited recovery capacity.
    • Three lines exhibited higher survival fractions and considerable low dose-rate sparing, with repair half times ranging from 0.25 to 5.7 hours.

    Conclusions:

    • Substantial heterogeneity in intrinsic radiation recovery capacity exists within carcinoma of the cervix.
    • These intrinsic differences highlight the potential clinical utility of predictive radiosensitivity testing for this disease.
    • Further research into predictive biomarkers could personalize cervical cancer radiotherapy.