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Application of effect-based methods (EBMs) in a river basin: a preliminary study in Central Italy
Walter Cristiano1, Ines Lacchetti1, Kevin Di Domenico1
1Unità Ecosistemi e Salute, Dipartimento Ambiente e Salute, Istituto Superiore di Sanità, Rome, Italy.
Annali Dell'Istituto Superiore Di Sanita
|April 4, 2020
Summary
Effect-based methods (EBMs) effectively detected chemical pollutants in the Tiber River using Daphnia magna and zebrafish bioassays. The Daphnia immobilization test yielded stronger results, highlighting EBMs for water quality monitoring.
Area of Science:
- Environmental Science
- Ecotoxicology
- Aquatic Ecology
Background:
- Effect-based methods (EBMs) offer innovative approaches for detecting environmental chemical pollutants.
- In vivo bioassays using freshwater species are crucial for assessing ecosystem health.
Purpose of the Study:
- To evaluate the water quality of the Tiber River ecosystem.
- To implement two in vivo bioassays on Daphnia magna and Danio rerio.
Main Methods:
- Daphnia sp. Acute Immobilisation assay and Fish Embryo Acute Toxicity (FET) test were employed.
- Water samples were collected from two urban sites on the Tiber River and one reference site on the Farfa River.
Main Results:
- The Fish Embryo Acute Toxicity (FET) test showed higher sensitivity in detecting low-level effects.
- The Daphnia magna Acute Immobilisation test provided the strongest statistically significant results.
Conclusions:
- EBMs are effective tools for investigating and monitoring chemical water pollution.
- Further research, including chemical analyses and additional bioassays, is needed for the Tiber River basin.
- Study findings will aid authorities in implementing pollution reduction measures.
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