Related Experiment Video
Updated: Dec 25, 2025
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
Live cell fluorescent stain of bacterial curli and biofilm through supramolecular recognition between bromophenol
Lixia Zhang1, Zhenhua Li, Zhijun Chen
1State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, College of Chemistry and International Joint Research Laboratory of Nano-Micro Architecture Chemistry, Jilin University, Changchun 130012, China. zchen@jlu.edu.cn.
Abstract:
Identification of curli-specific dyes for biofilm communities of microorganisms is an important task. We describe here a curli fluorescent light-up probe called bromophenol blue, which binds to curli via recognizing CsgA. This platform may provide a new perspective for the research on biofilm, amyloid disease and living materials.
