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Updated: Dec 24, 2025

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
Published on: November 1, 2014
De novo transcriptome assembly and data for the blue-winged teal (Spatula discors)
Amanda C Dolinski1, Jared J Homola1, Mark D Jankowski1,2
1Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI, United States.
Abstract:
The blue-winged teal (Spatula discors) is a recreationally and ecologically important dabbling duck species in North America. Transcriptomic data of this species can be used in public and animal health studies given its role as a natural reservoir host for avian influenza, which can be a zoonotic disease of high concern. Ileum and bursa of Fabricius tissues were sampled from six captive raised blue-winged teals, four of the six who were experimentally infected with low-pathogenic avian influenza virus H5N9. RNAseq data were generated from extracted total mRNA from each tissue and pooled to create a de novo assembly of the transcriptome using Trinity. A total of 571,105 transcripts were identified at 449,956 unique unigenes that have been functionally annotated. This transcriptome will be useful for future blue-winged teal gene expression research, especially in hypothesis driven differential expression studies to determine the driving forces of avian influenza host-pathogen interactions, spatial distribution, and transmission.

