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Multifunctional P(PEGMA)-REDV conjugated titanium surfaces for improved endothelial cell selectivity and
Yang Liu1, Timothy Thatt Yang Tan, Shaojun Yuan
1School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459 - Singapore. tytan@ntu.edu.sg.
Journal of Materials Chemistry. B
|April 9, 2020
Summary
This study developed a multifunctional titanium surface that selectively attracts endothelial cells (ECs) for better tissue engineering. The surface also maintains hemocompatibility, crucial for implantable devices.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Surface Chemistry
Background:
- Pre-vascularization with endothelial cells (ECs) and hemocompatibility are key for tissue-engineered construct viability.
- Current methods face challenges in achieving simultaneous EC selectivity and hemocompatibility on implant surfaces.
Purpose of the Study:
- To create a multifunctional titanium (Ti) surface that enhances EC attachment and selectivity while maintaining hemocompatibility.
- To evaluate the efficacy of REDV peptide conjugation on PEGMA polymer brushes for EC targeting on a modified Ti surface.
Main Methods:
- Surface modification of Ti using dopamine (DOPA) followed by surface-initiated atom transfer radical polymerization (ATRP) of PEGMA.
- Conjugation of REDV peptide onto the PEGMA polymer brushes.
- In vitro assessment of endothelial cell (EC) and mesenchymal stem cell (MSC) attachment and proliferation.
- Platelet adhesion assays to evaluate hemocompatibility.
Main Results:
- P(PEGMA)-REDV conjugation significantly improved human umbilical vein endothelial cell (HUVEC) proliferation and attachment compared to pristine Ti.
- REDV conjugation demonstrated selectivity for ECs, with no significant effect on mesenchymal stem cell (MSC) behavior.
- Immobilization of PEGMA polymer brushes enhanced surface hemocompatibility, an effect preserved after REDV conjugation.
Conclusions:
- The multifunctional Ti surface promotes selective EC attachment and proliferation, crucial for vascularization in tissue engineering.
- The developed surface exhibits improved hemocompatibility, making it suitable for biomedical applications.
- This surface modification strategy holds potential for bone and dental tissue engineering applications requiring enhanced vascularization and biocompatibility.
