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Published on: March 6, 2019
Disulfide-phenylazide: a reductively cleavable photoreactive linker for facile modification of nanoparticle surfaces
Qi Huang1, Chunyan Bao, Yao Lin
1Shanghai Key Laboratory of Functional Materials Chemistry, Institute of Fine Chemicals, East China University of Science and Technology, 130# Meilong Road, Shanghai, 200237, China. baochunyan@ecust.edu.cn linyongzhu@ecust.edu.cn.
Abstract:
In this study, a reductively cleavable photoreactive linker, disulfide-phenylazide (SPA), was synthesized and used to functionalize inorganic mesoporous silica nanoparticles (MSNs) by simple amidation reaction. Changed zeta potential, FT-IR and UV-vis or fluorescence spectra confirmed the successful photo-responsive functionalization of dextran (DSPA-MSNs) and immobilization of model avidin protein (ASPA-MSNs). With treatment by reducing agents, both DSPA-MSNs (with 10 mM DTT) and ASPA-MSNs (with 10 mM GSH) exhibited the reductive-responsive controllable release of coumarin 460 and bioactive protein. These results implied that the designed photo-affinity functional agent SPA with reductive-responsive cleavage provided a promising method to functionalize inorganic nanoparticles to improve their biocompatibility and biomedical applications.
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