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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Related Experiment Video

Updated: Dec 24, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Round Spermatid Injection.

Kelli X Gross¹, Brent M Hanson², James M Hotaling³

¹Division of Urology, Department of Surgery, University of Utah, 50 North Medical Drive, Salt Lake City, UT 84132, USA.

The Urologic Clinics of North America

|April 11, 2020

Summary

This summary is machine-generated.

Men with azoospermia face limited fertility options. Round spermatid injection (ROSI) offers a potential alternative by using immature sperm cells for fertilization when mature sperm are unavailable.

Keywords:

Assisted reproductive technology Azoospermia In vitro fertilization Male factor infertility Round spermatid injection Testicular sperm extraction

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Area of Science:

Reproductive Medicine and Endocrinology
Human Assisted Reproduction Technologies

Background:

Azoospermia presents significant fertility challenges for affected men.
Conventional testicular sperm extraction may fail to yield mature spermatozoa.
Limited options like adoption or donor sperm exist for these patients.

Purpose of the Study:

To explore the potential of round spermatid injection (ROSI) as a treatment for azoospermia.
To evaluate round spermatids as an alternative to mature spermatozoa in assisted reproduction.

Main Methods:

Utilized round spermatids, immature sperm cells, for injection into human oocytes.
Applied the technique known as round spermatid injection (ROSI).

Main Results:

Successful fertilization has been reported using round spermatids.
ROSI can serve as an alternative to mature spermatozoa for fertilization.

Conclusions:

Round spermatid injection (ROSI) shows potential as a treatment for male infertility due to azoospermia.
Despite limitations and reduced success rates, ROSI warrants consideration for specific patient groups.