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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

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Proteolistics: A Protein Delivery Method.

Susana Martin-Ortigosa^1,2, Kan Wang³

¹Department of Agronomy and Crop Bioengineering Center, Iowa State University, Ames, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 12, 2020

Summary

This summary is machine-generated.

Researchers developed "proteolistics," a method for delivering proteins into plant cells using biolistics. This technique enables transgene-free genome editing in plants by directly introducing proteins or protein/RNA complexes.

Keywords:

Biolistics Gene-gun Intracellular delivery Protein delivery Proteolistics

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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Published on: September 2, 2015

Area of Science:

Plant biology
Molecular biology
Biotechnology

Background:

Intracellular protein delivery is crucial for plant research and genome editing.
Current methods can lead to transgene integration, complicating research.
There is a need for efficient, transgene-free protein delivery systems in plants.

Purpose of the Study:

To describe a novel protocol for intracellular protein delivery in plant cells.
To enable transgene-free genome editing in plants.
To introduce a biolistics-based method termed 'proteolistics'.

Main Methods:

A protocol for delivering a protein/gold particle mixture into plant cells via biolistics.
Drying a protein/gold suspension directly onto a gene-gun cartridge or macrocarrier.
Utilizing the Bio-Rad PDS-1000/He particle delivery device for bombardment.

Main Results:

Successful intracellular delivery of proteins into plant cells was achieved.
The described method, 'proteolistics,' facilitates protein/protein-RNA complex delivery.
This approach allows for the production of gene-edited plants without genomic transgene integration.

Conclusions:

Proteolistics offers an effective method for intracellular protein delivery in plants.
This technique is valuable for plant research, particularly for genome editing applications.
It provides a pathway to generate transgene-free edited plants, advancing the field.