Related Experiment Video
Updated: Dec 24, 2025
09:45
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
Published on: February 4, 2011
27.9K
Separation, Characterization, and Handling of Microalgae by Dielectrophoresis
Vinzenz Abt1, Fabian Gringel2, Arum Han3
1Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Ackerstr. 76, ACK24, 13355 Berlin, Germany.
Microorganisms
|April 15, 2020
Summary
Dielectrophoresis (DEP) microfluidics offer label-free cell handling and characterization for microalgae. This review explores DEP applications in microalgae bioproduction, aiding researchers in selecting suitable methods.
Area of Science:
- Biotechnology
- Microfluidics
- Cell Biology
Background:
- Microalgae biotechnology holds potential for sustainable biomolecule production.
- Limited rapid cell characterization methods hinder industrial microalgae applications.
- Dielectrophoresis (DEP) offers label-free cell handling and characterization.
Purpose of the Study:
- To comprehensively review DEP-based microfluidic devices for microalgae.
- To explore DEP applications in microalgae handling, characterization, and separation.
- To provide insights for researchers on selecting appropriate DEP methods for microalgae bioproduction.
Main Methods:
- Review of existing literature on dielectrophoresis-based microfluidic devices.
- Analysis of DEP principles based on dielectric properties and cell size.
- Focus on applications in microalgae handling, characterization, and separation.
Main Results:
- DEP microfluidics enable label-free manipulation of microalgae.
- DEP effectiveness is influenced by changes in microalgal dielectric properties during bioproduction.
- DEP shows promise for separating microalgae based on intrinsic properties.
Conclusions:
- DEP-based microfluidics are valuable tools for advancing microalgae biotechnology.
- This review provides guidance for researchers to implement DEP in their studies.
- Further research in DEP can benefit cell-based bioproduction and industrial microalgae applications.
Related Concept Videos
01:30Capillary Electrophoresis: Applications
944
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
944
01:20Overview Of Cell Separation And Isolation
7.0K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.0K
01:20Electrophoresis: Overview
3.4K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
3.4K
02:35DNA Agarose Gel Electrophoresis
111.4K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
111.4K
01:20Capillary Electrophoresis: Instrumentation
755
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
755