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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
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A multipedal DNA walker for amplified detection of tumor exosomes
1CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. China. miaopeng@sibet.ac.cn pm3084@nyu.edu.
Abstract:
We have fabricated an ultrasensitive exosome biosensor based on a multipedal DNA walker with a core of target exosomes. Specific recognition is achieved by introducing a CD63 aptamer and facile separation is obtained with magnetic Fe3O4@Au nanoparticles. A limit of detection down to 6/μL is obtained with excellent selectivity.

