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Updated: Dec 24, 2025
Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
Published on: January 30, 2021
Can Keratin Scaffolds be used for Creating Three-dimensional Cell Cultures?
Marta Bochynska-Czyz1, Patrycja Redkiewicz1, Hanna Kozlowska2
1Department of Neuropeptides, Mossakowski Medical Research Centre Polish Academy of Science, 02-106 Warsaw, 5 Pawinskiego Street, Poland.
Abstract:
Three-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.
