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Related Experiment Videos

Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis.

R F Hector1, B L Zimmer, D Pappagianis

  • 1Cutter Biologicals, Berkeley, California 94701.

Journal of Clinical Microbiology
|December 1, 1988
PubMed
Summary

A new microtiter method enables antifungal susceptibility testing for Coccidioides immitis. This approach accurately assesses the efficacy of azole and nikkomycin drugs against the spherule-endospore phase of this fungus.

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Area of Science:

  • Mycology
  • Infectious Diseases
  • Antifungal Drug Discovery

Background:

  • Coccidioides immitis causes Valley fever, a significant fungal infection.
  • Accurate susceptibility testing is crucial for effective antifungal treatment.
  • Current methods for Coccidioides immitis susceptibility testing can be cumbersome.

Purpose of the Study:

  • To develop a standardized microtiter-based method for Coccidioides immitis antifungal susceptibility testing.
  • To evaluate the efficacy of azole and nikkomycin antifungals against Coccidioides immitis in vitro.

Main Methods:

  • A microtiter plate format was utilized for susceptibility testing.
  • Isolated spherule-endospore-phase Coccidioides immitis were inoculated into wells with modified Converse medium.

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  • Wells contained varying concentrations of azole or nikkomycin antifungal agents.
  • Plates were incubated at 37°C with shaking for 96 hours.

    • Main Results:

    • Visible turbidity and discernible endpoints were observed in control wells after incubation.
    • Microscopic examination confirmed that cells predominantly remained in the spherule-endospore phase in both control and treated wells.
    • The method allowed for the assessment of antifungal activity at different drug concentrations.

    Conclusions:

    • The developed microtiter method is effective for Coccidioides immitis antifungal susceptibility testing.
    • This assay supports the evaluation of novel antifungal agents against Coccidioides immitis.
    • The method maintains the fungal cells in the relevant spherule-endospore phase for testing.