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Published on: July 7, 2017
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Karolinska Institutet Human Embryonic Stem Cell Bank.
Heather Main1, Mona Hedenskog1, Ganesh Acharya1
1Department of Clinical Sciences, Intervention and Technology, Karolinska Insitutet, 17177, Stockholm, Sweden; Gynecology and Reproductive Medicine, Karolinska University Hospital, 14186 Stockholm, Sweden.
Stem Cell Research
|April 28, 2020
Summary
The Karolinska Institutet Human Embryonic Stem Cell Bank has developed 60 human embryonic stem cell (hESC) lines, including a clinical-grade line (KARO1), for research and potential therapeutic applications.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Biobanking
Background:
- The Karolinska Institutet Human Embryonic Stem Cell Bank (KI Stem Cell Bank) was founded in 2002.
- The bank's primary objective has been to derive human embryonic stem cell (hESC) lines suitable for clinical applications.
Purpose of the Study:
- To detail the establishment and growth of the KI Stem Cell Bank.
- To highlight progress towards generating clinical-grade hESC lines.
- To inform about the availability of hESC lines for research.
Main Methods:
- Derivation of human embryonic stem cell lines.
- Implementation of Good Manufacturing Practice (GMP) guidelines.
- Removal of xeno-components and establishment of chemically defined conditions.
Main Results:
- The KI Stem Cell Bank currently houses 60 hESC lines.
- A clinical-grade hESC line, KARO1, has been successfully derived and banked under GMP guidelines.
- hESC lines have been distributed to the scientific community and deposited in the Stockholm Medical Biobank.
Conclusions:
- The KI Stem Cell Bank has made significant strides in producing clinical-grade hESC lines.
- The bank's resources support both clinical translation and collaborative research efforts in the field of stem cell science.
