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Plasma sorbitol dehydrogenase determination in experimental hepatotoxicity using the Abbott Bichromatic Analyzer
A P Pakuts1, L W Whitehouse, C J Paul
1Bureau of Drug Research, Drug Toxicology Division, Tunney's Pasture, Ottawa, Ontario, Canada.
Abstract:
An automated single reagent micro-assay for the determination of sorbitol dehydrogenase (EC 1.1.1.14) catalytic activity concentration in mouse plasma was developed for the Abbott Bichromatic Analyzer (ABA-100). The kinetic rate determination was linear up to 250 U/l, had a CV of 2.65%, and required only 25 microliters of sample. Experimentally induced hepatotoxicity in the mouse by acetaminophen produced a dose dependent increase in blood sorbitol dehydrogenase activity.
