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Bioanalytical HPLC Applications of In-Tube Solid Phase Microextraction: A Two-Decade Overview
Natalia Manousi1, Paraskevas D Tzanavaras1, Constantinos K Zacharis2
1Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Molecules (Basel, Switzerland)
|May 6, 2020
Summary
In-tube solid phase microextraction (SPME) offers miniaturized, green, and automated sample preparation for analysis. This review covers twenty years of HPLC bioanalytical applications, materials, and instrumental aspects.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biotechnology
Background:
- In-tube solid phase microextraction (SPME) is an advanced sample preparation technique.
- It provides benefits like miniaturization, eco-friendliness, automation, and preconcentration.
- Research and publications on in-tube SPME have significantly increased.
Purpose of the Study:
- To review the High-Performance Liquid Chromatography (HPLC) bioanalytical applications of in-tube SPME.
- To cover a twenty-year span of research reports in this field.
- To discuss instrumental aspects of coupling in-tube SPME with HPLC.
Main Methods:
- Literature review of scientific publications on in-tube SPME and HPLC.
- Analysis of instrumental parameters for in-tube SPME-HPLC coupling.
- Compilation of data on materials, coatings, and applications in biological samples.
Main Results:
- Significant growth in in-tube SPME research and publications over two decades.
- Detailed overview of various HPLC bioanalytical applications using in-tube SPME.
- Information on materials, coatings, and specific uses in biological matrices.
Conclusions:
- In-tube SPME is a valuable and evolving technique for bioanalytical sample preparation.
- The coupling of in-tube SPME with HPLC is well-established and widely applied.
- Further advancements in materials and instrumentation are expected.
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