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Restoration of UPK1A-AS1 Expression Suppresses Cell Proliferation, Migration, and Invasion in Esophageal Squamous

Fang Du1, Tao Guo2, Chenghua Cao3

  • 1Department of Hematology and Oncology, No. 988 Hospital of Joint Logistic Support Force of the Chinese People's Liberation Army, Zhengzhou, Henan Province, People's Republic of China.

Cancer Management and Research
|May 6, 2020
PubMed
Abstract

Insights

Long non-coding RNA UPK1A antisense RNA 1 (UPK1A-AS1) suppresses esophageal squamous cell carcinoma (ESCC) progression. UPK1A-AS1 inhibits ESCC cell proliferation, invasion, and migration by sponging miR-1248.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized as critical regulators in cancer development.
  • Esophageal squamous cell carcinoma (ESCC) is a significant global health concern with complex molecular underpinnings.
  • The specific roles of many lncRNAs in ESCC pathogenesis remain to be elucidated.

Purpose of the Study:

  • To investigate the expression and function of lncRNA UPK1A antisense RNA 1 (UPK1A-AS1) in ESCC.
  • To determine the impact of UPK1A-AS1 on ESCC cell proliferation, invasion, and migration.
  • To elucidate the molecular mechanism involving UPK1A-AS1 and its interaction with miR-1248 in ESCC.

Main Methods:

  • Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for gene expression analysis.
  • Cell-based assays including CCK-8, colony formation, scratch-healing, and transwell invasion assays to assess cell behavior.
  • Luciferase reporter and RNA pull-down assays to confirm the interaction between UPK1A-AS1 and miR-1248.

Main Results:

  • UPK1A-AS1 was found to be downregulated in ESCC tissues and cell lines.
  • Overexpression of UPK1A-AS1 significantly inhibited ESCC cell proliferation, migration, and invasion.
  • UPK1A-AS1 directly targets and sponges miR-1248, indicating a regulatory feedback loop.

Conclusions:

  • UPK1A-AS1 functions as a tumor suppressor in ESCC.
  • The anti-cancer effects of UPK1A-AS1 are partially mediated through its interaction with miR-1248.
  • These findings offer new insights into the regulatory mechanisms governing ESCC progression and potential therapeutic targets.
Keywords:
UPK1A antisense RNA 1esophageal squamous cell carcinomalong non-coding RNAmiR-1248

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