BONE REPAIR AFTER THE GLASS-CERAMICS IMPLANTATION INTO THE RATS' FEMUR DEFECT

The aim is to study the bone response to the implantation of two glass-ceramic materials into the distal metaphysis of the femur of rats. Experiment was carried out on 30 white 4-month-old laboratory male rats. Animals were randomly divided into two groups for the implantation of two types of calcium silicate-phosphate glasses (BS-11 or ASZ-5) into the defect of femur. Histology analysis was performed 7, 14 and 30 days (n=5 in each group) after the surgery. Estimate of bone-to-implant area (BIC%), fibrous-tissue-to-implant area (FIC%) and collagen distribution in polarization light was performed. 7daysafterimplantationthe fibrous tissue and new-formed woven bone tissue were found around BS-11 and ASZ-5. After 7daysFIC% was greater than BIC%: in BS-11 group by 2.2 times (p<0.001) and in ASZ-5 group by 2.5 times (р<0.001). 14daysafterimplantation BIC% increased compared to 7 days: in BS-11 groupby 2.2 times (р<0.001) and in ASZ-5 groupby 2.7 times (р<0.001). After 30 days, the lamellar bone tissue was found around the implanted BS-11 and ASZ-5 (100% of the perimeter). ASZ-5 microparticles were embedded in the newly formed lamellar bone at 14 and 30 days after implantation. The collagen fibers were oriented parallel to the implants surface of BS-11 and ASZ-5. Glass-ceramic ASZ-5 and BS-11 materials have high biocompatibility, good osteoconductive and osteoinductive properties and are incorporated into the bone. However, the rapid degradation of ASZ-5 makes it fragile, which may become a limitation for its use in loaded areas of the skeleton.