Recovery of astaxanthin from shrimp (Penaeus vannamei) waste by ultrasonic-assisted extraction using ionic...

In this study, an ultrasonic-assisted extraction using microemulsions was developed for the recovery of natural astaxanthin from shrimp waste. To select applicable solvent systems, the phase equilibrium, microstructure, and physical properties of the microemulsions were investigated. Then the effect of ultrasonic power, ultrasonic time, and microemulsion composition on the extraction efficiency (EE_Ast) and extraction yield (E_Ast) of astaxanthin were determined. Compared with organic solvents (ethanol, acetone, and dimethyl sulfoxide), the microemulsion contained tributyloctylphosphonium bromide ([P₄₄₄₈]Br), tributyloctylphosphonium trifluoroacetate ([P₄₄₄₈]CF₃COO), or tetrabutylphosphonium trifluoroacetate ([P₄₄₄₄]CF₃COO) resulted in significantly enhanced extraction of astaxanthin due to the stronger electrostatic interactions and hydrogen-bonding interactions. When ultrasonic-assisted extraction conditions were 50 W and 60 min, the highest EE_Ast and E_Ast reached 32.47 µg·g^-1 and 99% by using the IL-in-water microemulsion of [P₄₄₄₈]Br/(TX-100 + n-butanol)/water (0.13:0.25:0.62, w/w), respectively. The IL-based microemulsion is an adequate alternative to conventional methods in the extraction and recovery of astaxanthin from natural bioresources.