RuBisCO nano enzyme for mimicking CO₂ conversion system in plants

Area of Science:

• Biomimetic Chemistry
• Nanotechnology
• Enzyme Engineering

Background:

• Carbon dioxide (CO2) conversion is crucial for mitigating climate change.
• Biomimetic systems offer sustainable solutions for CO2 capture.
• Enzyme immobilization enhances catalytic efficiency and stability.

Purpose of the Study:

• To develop and investigate a novel CO2 capture and conversion system.
• To synthesize nano ribulose bisphosphate carboxylase/oxygenase (RuBisCO) enzyme using the ANADOLUCA method.
• To evaluate the effectiveness of nano RuBisCO for CO2 conversion into 3-phosphoglycerate (3-PGA).

Main Methods:

• Synthesis of 93 nm nano RuBisCO enzyme via the ANADOLUCA method.
• Spectrophotometric determination of enzymatic activity by monitoring 3-PGA formation at 340 nm.
• Comparative analysis of nano and free RuBisCO activity under varying substrate concentrations, pH, temperature, and Mg2+ levels.

Main Results:

• Nano RuBisCO enzyme effectively catalyzes the conversion of CO2 to 3-PGA.
• Optimized reaction conditions were identified for nano RuBisCO.
• The nano RuBisCO enzyme exhibited significant reusability without structural degradation.

Conclusions:

• The developed nano RuBisCO enzyme is a promising candidate for CO2 capture and conversion systems.
• The ANADOLUCA method provides an effective route for enzyme immobilization.
• The reusability and stability of nano RuBisCO support its application in sustainable CO2 management.