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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

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Related Experiment Video

Updated: Dec 21, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Alternative Splicing in Hepatocellular Carcinoma.

Seung Eun Lee¹, Karel P Alcedo², Hong Jin Kim³

¹Department of Surgery, Chung-Ang University, Seoul, Korea; Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.

Cellular and Molecular Gastroenterology and Hepatology

|May 12, 2020

Summary

Alternative splicing significantly impacts hepatocellular carcinoma (HCC) development by altering gene expression. Understanding these splicing changes offers new avenues for HCC diagnosis and treatment.

Keywords:

Cancer Metabolism Variants mRNA

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Last Updated: Dec 21, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Hepatocellular carcinoma (HCC) is a major cause of cancer death globally, with poor survival rates.
Chronic liver disease progression to HCC involves complex molecular mechanisms.
Alternative splicing plays a critical role in regulating gene expression and protein diversity.

Purpose of the Study:

To review the role of alternative splicing in HCC pathogenesis.
To highlight key splicing factors, RNA binding proteins, and signaling pathways involved in HCC.
To discuss recent research on alternative splicing in liver cancer.

Main Methods:

Review of primary research from the past 3-5 years.
Analysis of large-scale transcriptomic data in liver cancer.
Focused mechanistic studies on alternative splicing in HCC.

Main Results:

Alternative splicing globally alters genes involved in metabolism, proliferation, and apoptosis in HCC.
Splicing factors and RNA binding proteins are key regulators of HCC progression.
Dysregulated alternative splicing provides potential prognostic markers and therapeutic targets.

Conclusions:

Alternative splicing is a critical driver of HCC.
Further research into splicing mechanisms can lead to improved HCC diagnosis and management.
Targeting alternative splicing pathways may offer novel therapeutic strategies for HCC.