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Updated: Dec 21, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

A STIMulating journey into optogenetic engineering.

Guolin Ma¹, Yubin Zhou¹

¹Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA.

|May 14, 2020

Summary

This summary is machine-generated.

Genetically-encoded calcium actuators (GECAs) derived from STIM1 allow for optical control of ORAI1 channels. This technology provides a non-invasive method to study calcium channel function and control animal behavior wirelessly.

Keywords:

CRAC channel Calcium signaling Optogenetics STIM1

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In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Area of Science:

Molecular Biology
Neuroscience
Biophysics

Background:

Calcium signaling is crucial for cellular functions in both excitable and non-excitable tissues.
ORAI1 channels are key players in store-operated calcium entry.
Existing methods for manipulating calcium channels can be invasive.

Purpose of the Study:

To introduce and characterize genetically-encoded calcium actuators (GECAs) derived from STIM1.
To demonstrate the optical activation of endogenous ORAI1 channels using GECAs.
To explore the utility of GECAs for probing calcium channel structure-function relationships and controlling animal behavior.

Main Methods:

Genetic engineering to create GECAs based on STIM1.
Optical stimulation techniques.
Electrophysiological recordings in various tissue types.
Behavioral studies in awake, behaving mice.

Main Results:

GECAs successfully enabled optical activation of endogenous ORAI1 channels.
This activation was demonstrated in both excitable and non-excitable cell types.
GECAs provided a non-invasive tool to investigate ORAI1 channel function.
Wireless control of awake mouse behavior via GECAs was achieved.

Conclusions:

GECAs represent a novel, non-invasive technology for studying calcium channel biology.
This approach offers significant potential for understanding calcium channelopathies and developing new therapeutic strategies.
The ability to wirelessly control behavior opens new avenues for neuroscience research.