Exposure of synthesized Co₃O₄ nanoparticles to Chlorella minutissima: An ecotoxic evaluation in freshwater microalgae

The current study focuses on the ecotoxicity of cobalt oxide nanoparticles (Co₃O₄ NPs) in the aquatic environment towards freshwater microalgae, Chlorella minutissima. The interaction of Co₃O₄ NPs with microalgae shows the growth suppressing effect. The 72 h EC ₅₀ (effective concentration of a chemical having 50% of its impact) values of Co₃O₄ NPs for C. minutissima was 38.16 ± 1.99 mg/L. The decline in chlorophyll a content and increase in reactive oxygen species (ROS) also indicated the compromised physiological state of microalgae. An increased LDH (lactate dehydrogenase) level in treated samples suggests membrane disintegration by Co₃O₄ NPs. Light microscopy, scanning electron microscopy (SEM) and Energy Dispersive X-Ray-Scanning electron microscopy (EDX-SEM) further confirm cell entrapment and deposition of Co₃O₄ NPs on the cell surface. Cellular internalization of NPs, as shown by Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES), also contributes towards the toxicity of NPs. The findings suggest the role of extracellular as well as intracellular nanoparticles (NPs) in exerting a toxic effect on the C. minutissima.