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Updated: Dec 21, 2025
An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production
Published on: March 27, 2017
Uniform straw-like cell architecture for three-dimensional cell-cell communication assay
Yusaku Inubushi1, Yoshiki Sakaguchi1, Akira Tachibana1
1Department of Bioengineering, Graduate School of Engineering, Osaka City University , Osaka, Japan.
Abstract:
We fabricated uniform straw-like cell architecture with central lumen using a suture thread within 1 h. The architecture consisting of cancer cells and mature adipocyte was used for cell-cell communication assay, although mature adipocyte could not form spontaneous multi-cellular spheroids. Using the system, it is possible to investigate three-dimensional cell-cell communication as an alternative to animal experiments.
Abbreviations:
EB: embryoid body; 2D: two-dimension; 3D: three-dimension; PBS: phosphate buffered saline.
