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Updated: Dec 20, 2025

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Cytochalasans from endophytic fungus Diaporthe sp. SC-J0138
Xiaoli Yang1, Ping Wu2, Jinghua Xue2
1Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, People's Republic of China; University of Chinese Academy of Sciences, Yuquanlu 19A, Beijing 100049, People's Republic of China.
Abstract:
Five new cytochalasans, diaporthichalasins D-H (1-5), along with five known cytochalasans (6-10) were isolated from solid cultures of the endophytic fungus Diaporthe sp. SC-J0138 isolated from the leaves of Cyclosorus parasiticus. Their structures were elucidated by analysis of spectroscopic data and theoretical calculations of electronic circular dichroism spectra. Compounds 1 and 5 showed noticeable cytotoxicity against human carcinoma A549, HeLa, and HepG2 cells. The structure-activity relationships in cytotoxicity were discussed for this group of compounds.
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