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Updated: Dec 20, 2025

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Management of Post-DALK Recipient Posterior Stromal Scarring: A Novel Approach
Vijay K Sharma1, Hemant S Trehan, Raji Kurumkattil
1Department of Ophthalmology, Army Hospital Research and Referral, New Delhi.
Aim:
Management of recipient posterior stromal scarring following deep anterior lamellar keratoplasty (DALK) by a novel approach.
Methods:
A side port knife bent at the tip is used to make a linear incision in the host posterior stroma. Plane between host posterior stroma and DALK tissue is separated using a reverse sinskey hook and multiple radial incisions are made using microvitroretinal scissors. The recipient posterior stromal wedges are removed using 20G vitrector with a cut rate of 800 cuts/min. A Descemet stripping endothelial keratoplasty lenticule is inserted into the anterior chamber and apposed to the posterior surface of DALK tissue using air tamponade.
Result:
This technique was used in 10 patients who showed clearing of the scarring after the surgery with good apposition of the donor graft to the previous DALK tissue. Conclusion: This is a sutureless, relatively simple, inexpensive, and effective novel surgical technique that avoids complications of open sky procedure and can be replicated in any ophthalmologic operation theatre setup.
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