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Updated: Dec 20, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
CRISPR-Cas9 system: A genome-editing tool with endless possibilities.
Swati Tyagi1, Robin Kumar2, Aparup Das3
1Genomic Division, National Institute of Agriculture Science, Rural Development Administration, Jeonju, Republic of Korea.
CRISPR-Cas9 gene editing offers powerful applications in biology, accelerating advancements in agriculture and medicine. However, careful consideration of its limitations and ethical implications is crucial for responsible societal integration.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- The CRISPR-Cas9 system has revolutionized gene editing, providing a versatile tool for modifying genomes across diverse organisms.
- Its efficiency and flexibility allow for precise genetic alterations in prokaryotic and eukaryotic systems, including plants and animals.
Purpose of the Study:
- To review the beneficial applications of the CRISPR-Cas9 gene-editing system.
- To discuss the potential risks and societal perceptions associated with its misuse.
- To explore the limitations and ethical considerations surrounding CRISPR-Cas9 technology.
Main Methods:
- Literature review of CRISPR-Cas9 applications and implications.
- Analysis of current research trends in gene editing.
- Discussion of ethical frameworks and societal concerns.
Main Results:
- CRISPR-Cas9 facilitates advancements in crop and livestock breeding, antimicrobial engineering, and pathogen control.
- The technology presents significant societal challenges and safety concerns if not handled with care.
- Identified limitations and ethical dilemmas require careful consideration for responsible implementation.
Conclusions:
- CRISPR-Cas9 is a transformative technology with broad scientific and practical benefits.
- Addressing its limitations and ethical issues is paramount for safe and beneficial societal integration.
- Continued research and open discussion are essential for navigating the future of gene editing.
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