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Related Experiment Videos

Processing of quantitative scanning transmission electron micrographs.

A Engel1, R Reichelt

  • 1M.E. Müller-Institute for High-resolution Electron Microscopy at the Biocenter, University of Basel, Switzerland.

Scanning Microscopy. Supplement
|January 1, 1988
PubMed
Summary
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Scanning transmission electron microscopy (STEM) offers quantitative structural insights into biological molecules. New methods enable mass and element mapping for detailed macromolecular analysis.

Area of Science:

  • Structural biology
  • Biophysics
  • Electron microscopy

Background:

  • Digital image processing is common for 3-D reconstruction in electron microscopy (EM).
  • Scanning transmission electron microscopy (STEM) offers unique quantitative analysis of biological structures.
  • STEM can determine mass and elemental distribution within macromolecules.

Purpose of the Study:

  • To develop STEM-specific procedures for quantitative analysis of biological macromolecules.
  • To enable mass and elemental distribution mapping within biological structures.

Main Methods:

  • Utilizing STEM elastic darkfield (DF) micrographs for high-resolution structural definition.
  • Averaging multiple STEM DF micrographs from regular structures to create mass maps.

Related Experiment Videos

  • Analyzing weaker signals from electron energy loss windows for elemental mapping.

    • Main Results:

    • STEM elastic DF imaging allows for high-resolution structural averaging.
    • Mass maps can reveal structural domains as small as 1,000 daltons.
    • Averaging signals from electron energy loss windows is expected to yield element maps.

    Conclusions:

    • STEM provides powerful quantitative methods for analyzing biological macromolecular structure.
    • Combined elastic DF and electron energy loss analysis can reveal detailed mass and elemental composition.
    • These techniques advance the understanding of macromolecular structure and function.