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High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules.

Silvia Pisani1, Rossella Dorati2, Ida Genta2

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|May 28, 2020
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Summary

High efficiency vibrational technology (HEVT) successfully produced poly(methyl-methacrylate) (PMMA) microcapsules for cell encapsulation. These biocompatible microcapsules support cell survival and growth, showing promise for tissue regeneration applications.

Keywords:
cell microencapsulationfibroblastshigh efficiency vibrational technologypoly(methyl-methacrylate

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Poly(methyl-methacrylate) (PMMA) is a biocompatible, non-biodegradable polymer frequently utilized in biomedical applications.
  • Microencapsulation of live cells within polymers is a key strategy for tissue regeneration.
  • Developing efficient methods for producing cell-loaded microcapsules is crucial for advancing regenerative medicine.

Purpose of the Study:

  • To assess the feasibility of using high efficiency vibrational technology (HEVT) for producing poly(methyl-methacrylate) (PMMA) microcapsules loaded with live cells.
  • To optimize process parameters for creating PMMA microcapsules suitable for cell encapsulation and tissue regeneration.
  • To evaluate the characteristics, permeability, and cell viability within the produced microcapsules.

Main Methods:

  • Preliminary studies were conducted to optimize HEVT parameters for PMMA microcapsule production.
  • Human dermal fibroblasts were encapsulated within shell/core PMMA microcapsules.
  • Morphometric analysis using optical and scanning electron microscopy was performed.
  • Permeability studies assessed the diffusion of molecules with varying molecular weights.
  • Cell viability and growth were monitored over 72 hours in a simulated physiological medium.

Main Results:

  • HEVT produced uniform 1.2 mm PMMA microcapsules with circular surface pores under optimized conditions (200 Hz, 750 V, 10 mL/min core flow, 0.5 bar shell flow).
  • The microcapsule membrane allowed permeation of molecules up to 5900 Da but blocked larger molecules (11,000 Da).
  • The process achieved a yield of approximately 50% with a cell encapsulation efficiency of 27%.
  • Encapsulated human dermal fibroblasts demonstrated survival and growth for up to 72 hours.

Conclusions:

  • High efficiency vibrational technology (HEVT) is a viable method for producing cell-loaded PMMA microcapsules.
  • The developed PMMA microcapsules possess suitable characteristics for encapsulating cells for tissue regeneration.
  • Encapsulated cells remain viable and grow, indicating the potential of these microcapsules in regenerative medicine strategies.