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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
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TADF Dye-Loaded Nanoparticles for Fluorescence Live-Cell Imaging.

Carina I C Crucho1, João Avó1, Ana M Diniz1

  • 1IBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.

Frontiers in Chemistry
|May 28, 2020
PubMed
Summary
This summary is machine-generated.

Thermally activated delayed fluorescence (TADF) emitters were encapsulated in polymeric nanoparticles, enabling their use in live-cell imaging. This approach overcomes solubility issues and maintains luminescence in aqueous environments for biological applications.

Keywords:
TADFdye-loaded nanoparticlesfluorescence imagingfluorescence microscopyluminescent probesoptical imaging

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Area of Science:

  • Materials Science
  • Chemistry
  • Biotechnology

Background:

  • Thermally activated delayed fluorescence (TADF) molecules are crucial for efficient organic light-emitting diodes, utilizing triplet states without heavy metals.
  • TADF emitters exhibit a small singlet-triplet energy gap, enabling reverse intersystem crossing for delayed fluorescence.
  • Current limitations for TADF in biological applications include poor biocompatibility, low aqueous solubility, and reduced performance in polar media.

Purpose of the Study:

  • To develop a method for utilizing TADF emitters in biological imaging by overcoming their limitations in polar media.
  • To create novel TADF-emitting nanomaterials suitable for live-cell imaging applications.
  • To demonstrate the preservation of TADF photophysical properties in aqueous environments using polymeric nanoparticles.

Main Methods:

  • Two established TADF emitters were loaded onto polystyrene nanoparticles via a swelling procedure.
  • The resulting TADF-emitting nanomaterials were characterized using optical spectroscopy.
  • The cytotoxicity and cellular uptake of the nanoparticles were evaluated in live human cancer cells.

Main Results:

  • The synthesized nanoparticles exhibited characteristic TADF emission in aqueous media, with the polymeric matrix protecting the dyes from solvent effects.
  • The TADF nanomaterials demonstrated very low cytotoxicity towards live human cancer cells.
  • Effective cellular uptake allowed for fluorescence microscopy imaging at low concentrations.

Conclusions:

  • Polymeric nanoparticles provide a versatile platform for adapting TADF emitters for biological applications, maintaining their photophysical properties.
  • The developed TADF nanomaterials are suitable for live-cell imaging due to their biocompatibility, aqueous stability, and efficient cellular uptake.
  • This facile method offers a generalized approach for creating TADF-based bio-imaging tools.