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Related Experiment Video

Updated: Dec 20, 2025

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

Intelligent image-activated cell sorting 2.0.

Akihiro Isozaki¹, Hideharu Mikami, Hiroshi Tezuka

¹Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan. goda@chem.s.u-tokyo.ac.jp.

|May 28, 2020

Summary

This summary is machine-generated.

A new intelligent image-activated cell sorting (iIACS) machine offers 20x higher throughput and sensitivity. This advanced cell sorting technology utilizes virtual-freezing fluorescence imaging and real-time AI processing for broader biological applications.

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Area of Science:

Biotechnology
Microfluidics
Artificial Intelligence

Background:

Intelligent image-activated cell sorting (iIACS) integrates microscopy, cell sorting, and deep learning for high-throughput analysis.
Existing iIACS systems have limitations in throughput and sensitivity, restricting their application scope.

Purpose of the Study:

To develop and report on a novel iIACS machine with significantly enhanced system performance.
To expand the capabilities and applications of image-based cell sorting technology.

Main Methods:

Development of an iIACS machine integrating a high-throughput fluorescence microscope, cell focuser, cell sorter, and deep neural network.
Implementation of virtual-freezing fluorescence imaging and a real-time intelligent image processor on an 8-PC server with multi-core CPUs and GPUs.
Characterization using fluorescent particles and diverse cell types to validate performance.

Main Results:

Achieved a throughput of approximately 2000 events per second, a 20-fold improvement over previous reports.
Reached a sensitivity of approximately 50 molecules of equivalent soluble fluorophores (MESFs), also a 20-fold improvement.
System performance closely matched achievable design specifications.

Conclusions:

The new generation iIACS technology demonstrates substantial advancements in throughput and sensitivity.
This enhanced iIACS machine is poised to drive discoveries across immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology.