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Updated: Dec 20, 2025

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Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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Targeted Quantification of Phosphopeptides by Parallel Reaction Monitoring (PRM)
Sara Christina Stolze1, Hirofumi Nakagami2
1Protein Mass Spectrometry Group, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2020
Abstract:
Parallel reaction monitoring (PRM) is a liquid chromatography-mass spectrometry (LC-MS)-based targeted peptide/protein quantification method that was initially implemented for Orbitrap mass spectrometers. Here, we describe detailed workflows that utilize the freely available MaxQuant and Skyline software packages to target peptides of interest, primarily focusing on phosphopeptides.

