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Updated: Dec 20, 2025

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
Published on: April 26, 2024
In Silico Analysis of Class III Peroxidases: Hypothetical Structure, Ligand Binding Sites, Posttranslational
Sabine Lüthje1, Kalaivani Ramanathan2
1Oxidative Stress and Plant Proteomics Group, Institute for Plant Science and Microbiology, University of Hamburg, Hamburg, Germany. Sabine.Luethje@uni-hamburg.de.
Abstract:
Functional analyses of peroxidases are a major challenge. In silico analysis appears to be a powerful tool to overcome at least some of the problems that arose from (1) the numerous possible functions of peroxidases, (2) their low substrate specificity, and (3) the compensation of knockout mutants by other isoenzymes. Amino acid sequences and crystal structures of peroxidases were used for the prediction of tertiary structures, posttranslational modifications, ligand and substrate binding sites, and so on of uncharacterized peroxidases. This protocol presents tools and their applications for an in silico analysis of soluble and membrane-bound peroxidases, but it may be used for other proteins, too.
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