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Enhanced sugar chain detection by oriented immobilization of Fc-fused lectins
Masumi Iijima1,2, Yuki Yamada1, Tsutomu Nakayama2
1The Institute of Scientific and Industrial Research, Osaka University , Ibaraki, Osaka, Japan.
Bioscience, Biotechnology, and Biochemistry
|June 2, 2020
Abstract:
We report a novel scaffold for clustering and oriented immobilization of human IgG1 Fc-fused lectins on biosensors without chemical modifications. This approach uses a bio-nanocapsule (BNC) displaying a tandem form of IgG Fc-binding Z domains derived from Staphylococcus aureus protein A (ZZ-BNC). Incorporating ZZ-BNC effectively increased both the sensitivity and sugar chain-binding capacity compared with the condition without ZZ-BNC.

