Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Charge-Based Discrimination of Amyloids Using an Amyloid-Targeting Chemiluminescent Probe.

Chembiochem : a European journal of chemical biology·2026

Same author

A NOD2-Encoded Toggle Switch Resolves the Host-Microbe Battle Over Cyclic AMP Control.

bioRxiv : the preprint server for biology·2026

Same author

A Druggable G Protein Checkpoint in Cholesterol Efflux.

bioRxiv : the preprint server for biology·2026

Same author

CANDiT: A machine learning framework for differentiation therapy in colorectal cancer.

Cell reports. Medicine·2025

Same author

Nootropic benzothiazoles promote dendritic spine formation by targeting fascin-1.

The Journal of biological chemistry·2025

Same author

Rurality and Outcomes of Patients Undergoing Mechanical Thrombectomy for Acute Ischemic Stroke.

Critical care research and practice·2025

Same journal

1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

Methods in enzymology·2026

Same journal

Nitrone dipoles in bioorthogonal chemistry applications.

Methods in enzymology·2026

Same journal

Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

Methods in enzymology·2026

Same journal

Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

Methods in enzymology·2026

Same journal

inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

Methods in enzymology·2026

Same journal

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 20, 2025

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

Method to discriminate amyloids using fluorescent probes.

Kristine L Teppang¹, Rachel S Ehrlich¹, Jerry Yang¹

¹Department of Chemistry and Biochemistry, University of California, San Diego, CA, United States.

Methods in Enzymology

|June 2, 2020

Summary

This summary is machine-generated.

This study introduces ANCA-11, a novel fluorescent probe for detecting amyloid aggregates. This probe differentiates amyloids by disease origin, aiding in neurodegenerative disease diagnosis.

Keywords:

Alzheimer's disease Amyloid-β Amyloids Discrimination Fluorescent probes

More Related Videos

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Related Experiment Videos

Last Updated: Dec 20, 2025

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Published on: October 20, 2017

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Area of Science:

Biochemistry
Neuroscience
Medical Diagnostics

Background:

Misfolded protein aggregation into amyloids is central to numerous neurodegenerative and non-neurologic diseases.
Targeted fluorescent probes are crucial for rapid amyloid detection and discrimination based on protein composition, aiding disease diagnosis.

Purpose of the Study:

To present protocols for synthesizing and utilizing ANCA-11, an environmentally-sensitive fluorescent probe.
To demonstrate ANCA-11's capability to differentiate amyloids originating from different diseases.
To provide a standardized protocol for preparing synthetic Amyloid-β(1-42) samples to streamline research.

Main Methods:

Synthesis and application protocols for the ANCA-11 fluorescent probe.
Development of a method for preparing synthetic Amyloid-β(1-42) samples.
Generalizable methods for evaluating other amyloid-targeting fluorescent probes with various amyloidogenic protein aggregates.

Main Results:

Successful synthesis and application of ANCA-11 demonstrated its utility as an amyloid-targeting probe.
ANCA-11 effectively discriminated between amyloids of different disease origins using fluorescence.
A reliable protocol for Amyloid-β(1-42) preparation was established, addressing common research challenges.

Conclusions:

ANCA-11 is a valuable tool for fluorescently discriminating between disease-specific amyloids.
The presented methods facilitate the evaluation of novel fluorescent probes for amyloid detection.
Standardized amyloid sample preparation enhances research efficiency and reproducibility in amyloid-related disease studies.