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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Dec 20, 2025

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

Simple Recommendations for Improving Efficiency in Generating Genome-Edited Mice.

O A Averina^1,2, M Y Vysokikh², O A Permyakov¹

¹Institute of functional genomics, Lomonosov Moscow State University, Moscow, 119991 Russia.

|June 2, 2020

Summary

This summary is machine-generated.

Optimizing CRISPR/Cas9 microinjection into mouse zygotes enhances genome editing efficiency. This study details techniques for efficiently generating knockout mouse strains for research applications.

Keywords:

genome editing mouse foster mothers mouse zygote donors superovulation transgenic mice

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Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

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Last Updated: Dec 20, 2025

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

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Published on: December 16, 2022

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

Area of Science:

Genetics and Genomics
Developmental Biology
Animal Models

Background:

Transgenic mouse models are crucial for biological research.
Efficient generation of genome-edited strains is essential for scientific advancement.

Purpose of the Study:

To optimize CRISPR/Cas9 microinjection techniques for efficient mouse genome editing.
To share practical experience in generating knockout mouse strains.

Main Methods:

Microinjection of CRISPR/Cas9 components into approximately 10,000 mouse zygotes.
Optimization of superovulation protocols.
Selection of optimal mouse strains for zygote donation and foster care.

Main Results:

Demonstrated improved efficiency in generating knockout mice through optimized techniques.
Identified key factors for successful genome editing in mouse zygotes.

Conclusions:

The presented methods offer a pathway for laboratories to rapidly and efficiently create custom genome-edited mouse strains.
Experience-based optimization significantly enhances the success rate of generating genetically modified mice.