Dentin Conditioning Protocol for Regenerative Endodontic Procedures.

Area of Science:

• Biomaterials Science
• Stem Cell Biology
• Endodontics

Background:

• Optimizing irrigation protocols is crucial for maintaining dental pulp stem cell (DPSC) viability and morphology.
• Sodium hypochlorite and EDTA are commonly used irrigants in endodontics.
• Investigating the effects of nanobubble (NB) water and ultrasonic activation on EDTA irrigation is essential.

Purpose of the Study:

• To optimize sodium hypochlorite-EDTA irrigation for DPSC viability and morphology.
• To evaluate the effects of an optimized EDTA protocol, alone or with NB water, on cell behavior.
• To assess the impact of ultrasonic activation on EDTA-conditioned dentin.

Main Methods:

• Human dentin discs were conditioned with various sodium hypochlorite and EDTA irrigation protocols.
• DPSC viability, morphology, and migration were assessed using cell viability assays, live-dead assays, and transwell migration assays.
• Transforming growth factor beta release was measured using ELISA.

Main Results:

• Phosphate-buffered saline (PBS) as a final rinse significantly improved DPSC viability on dentin.
• Ultrasonic-activated EDTA enhanced transforming growth factor beta release, DPSC viability, and migration compared to standard EDTA.
• EDTA prepared with NB water showed no significant difference in biological properties compared to regular EDTA.

Conclusions:

• Final rinsing with PBS effectively removes residual EDTA, enhancing DPSC viability.
• Ultrasonic activation of EDTA improves its biological properties and promotes cell behavior.
• Nanobubble water does not offer additional benefits when preparing EDTA solutions for endodontic irrigation.